Sale!

Universal Protein Extraction and Cleanup Kit – Peptides (UPECK-Pep)

$100.00$175.00

Launching 11/18/2019:  For a limited time get 50% off and free shipping, plus a free Amazon gift card when you fill out our feedback form after using our kit!  Only 30 kits available so pre-order today!

Our protein sample extraction, cleanup, and digestion kit produces peptide samples for mass spectrometry analysis in an easy-to-do workflow.  Works with any starting sample for fast,  high yield extraction, cleanup of protein samples, and digestion into peptides.

Each kit includes:

Strong lysis buffer

ProMTag protein tagging agent

ProMTag protein capture resin

Wash buffers

Reducing and alkylating reagents

Stabilized mP-trypsin

Mass spectrometry compatible and volatile elution buffer

Low protein binding tubes

Waste collection tubes

 

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SKU: Pep-1000 Category:

The UPECK-Pep kit produces high-quality peptide samples for mass spectrometry analysis in a fast, easy-to-do workflow from cell lysis all the way to peptides.  Works with any starting sample for fast,  high yield extraction, cleanup of protein samples, and digestion into peptides for mass spectrometry analysis.

Each kit includes:

Strong lysis buffer

ProMTag protein tagging agent

ProMTag protein capture resin

Wash buffers

Reducing and alkylating reagents

Stabilized mP-Trypsin

Mass spectrometry compatible and volatile elution buffer

Low protein binding tubes

Waste collection tubes

 

What you’ll need:

Tabletop centrifuge

Rotisserie or shaker

Heating block

 

How it works:

Our patent pending reversible chemistry allows for extraction, cleanup, and digestion in an fast, easy-to-perform workflow:

  1. Lyse cells or tissue with lysis buffer
  2. Label proteins with ProMTag
  3. Capture proteins with ProMTag resin
  4. Wash away contaminants
  5. Release proteins from ProMTag resin
  6. Reduce/alkylate proteins
  7. Digest proteins into peptide with stabilized ProMTag trypsin
  8. Capture ProMTag trypsin on ProMTag resin
  9. Elute clean, digested peptides

 

See more on our How It Works page

Download the quickstart protocol here

Download the full length protocol here

Download the Safety Data Sheet here

Starting protein amount per prep

5 – 40 ug, 41 – 200 ug

Starting protein concentration per prep

less than 1 mg/mL, 1 mg/mL or greater